Category: Biology Cells
Comparison of immunomagnetic beads coated with protein A, protein G, or goat anti-mouse immunoglobulins. Applications in enzyme immunoassays and immunomagnetic separations.
Immunomagnetic beads were prepared using either protein A (PA) or protein G (PG) coupled to magnetic beads for binding antibodies at their Fc region. The performance of these beads was compared with commercially available beads coated with goat anti-mouse (G alpha M) immunoglobulins. Both the PA- and PG-beads possessed a higher binding capacity than the…
Read MoreA hypothesis: Fructosamine-3-Kinase-Related-Protein (FN3KRP) catalyzes deglycation of Maillard intermediates directly downstream from fructosamines
Non-enzymatic glycation (a.k.a. Maillard reaction) is a series of random spontaneous reactions between reducing sugars and amines resulting in the formation of irreversible Advanced Glycation Endproducts (AGE’s). In food chemistry, this process is beneficial by contributing to flavor, aroma, texture and appearance of cooked foods. In vivo, however, Maillard reaction is deleterious because uncontrolled modification…
Read MoreEvaluation of the INDICAID COVID-19 Rapid Antigen Test in Symptomatic Populations and Asymptomatic Community Testing
As the COVID-19 pandemic progresses, there is an increasing need for rapid, accessible assays for SARS-CoV-2 detection. We present a clinical evaluation and real-world implementation of the INDICAID COVID-19 rapid antigen test (INDICAID rapid test). A multisite clinical evaluation of the INDICAID rapid test using prospectively collected nasal (bilateral anterior) swab samples from symptomatic subjects…
Read MoreEvaluating the genetic basis of anti-cancer property of Taxol in Saccharomyces cerevisiae model
Taxol has been regarded as one of the most successful anti-cancer drugs identified from natural sources to date. Although taxol is known to sensitize cells by stabilizing microtubules, its ability to cause DNA damage in peripheral blood lymphocytes, and to induce oxidative stress and apoptosis indicates that taxol may have other modes of cytotoxic action.…
Read MoreHigh-Throughput Screening of a Promoter Library Reveals New Persister Mechanisms in Escherichia Coli
Persister cells are a small subpopulation of phenotypic variants that survive excessive concentrations of bactericidal antibiotics. Their survival mechanisms will not be heritable and will be fashioned stochastically or triggered by environmental stresses equivalent to antibiotic therapy. On this examine, high-throughput screening of an Escherichia coli promoter library and subsequent validation experiments recognized a number…
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