Concavalin A

Concanavalin A Hepatitis

Concanavalin A (Con A) is a plant lectin that’s purified from jack beans. Con A binds to the mannose residues of assorted glycoproteins and prompts lymphocytes. When Con A is run to mice, liver damage that will depend on the activation of T lymphocytes by macrophages happens (Tiegs et al., 1992). Due to this fact, the mannequin may enable the examine of the pathophysiology of immunologically mediated hepatic issues, resembling AIH. TNF-α and IFN-γ play vital roles in Con A–induced liver damage (Gantner et al., 1995; Kusters et al., 1996) and IL-10 prevents liver damage on this mannequin (Di Marco et al., 1999; Louis et al., 1997). Blood ranges of IL-2, IL-4, and IFN-γ dramatically enhance after administration of Con A (Wang et al., 2012). Main drawbacks of this mannequin embrace the dearth of circulating autoantibodies and fast hepatocyte injury following a single-dose Con A injection, which isn’t a typical characteristic of persistent AIH.

 

Lectins as modulators of autophagy in most cancers immunotherapy

Concanavalin A prompts T cells and pure killer cells and triggers anticancer immune responses

Since ConA is a well known T cell mitogen it might additionally set off anticancer immune responses by activating tumor particular CTLs. Certainly, therapy of hepatoma-bearing mice (ML-14a cells) with ConA (7.5 mg/kg) strongly diminished liver tumor formation and extended survival, whereby 40% of the animals had been tumor free on the finish of the experiment [8]. Lymphocytic infiltrates round tumor nodules had been noticed in ConA-treated animals, suggesting T cell mediated responses. Certainly, the depletion of CD8+ T cells utterly abrogated the therapeutic results of ConA, whereas the depletion of CD4+ T cells solely partly prevented ConA-mediated inhibition of tumor formation (Fig. 4A). Consistent with this examine, ConA triggered CD4+ and CD8+ T cell activation in mice as proven by the upregulated expression of CD69 [74].

Concavalin A
Concanavalin A

As well as, ConA-treated mice contained elevated numbers of activated pure killer (NK) cells, T cells, and NK T cells, which confirmed robust cytotoxic exercise in opposition to murine lymphoma cells in ex vivo assays. That is additionally translated right into a strongly diminished variety of liver nodules upon ConA therapy of colon cancer-bearing mice (colon-26), an have an effect on that was abrogated upon NK cell depletion (Fig. 4A). Of observe, NK cells in immunodeficient nude mice (missing NKT and T cells) weren’t activated upon ConA therapy, suggesting a job of the adaptive immune system in activating NK cells [74].

 

Moreover, all results had been abrogated in IFNγ knock-out mice, displaying the significance of an lively immune response. Nevertheless, the therapy with ConA of hepatoma-bearing SCID mice additionally considerably inhibited liver tumor formation, however solely on the highest dose of 20 mg/kg [8]. As SCID mice lack purposeful lymphocytes, these information counsel a direct cytotoxic impact of ConA towards the most cancers cells. Certainly, ConA induced direct autophagy-dependent cell demise in these mice (see above paragraph about ConA and most cancers cell demise). Thus, ConA prompts CD8+ CTLs and NK cells that cut back in vivo tumor progress and potentiate ex vivo tumor cell lysis (Fig. 4A).

 

Recombinant Human Programmed cell death protein 10 Protein, His-SUMO, E.coli-100ug

QP8043-ec-100ug 100ug
EUR 489.6

Recombinant Human Programmed cell death protein 10 Protein, His-SUMO, E.coli-10ug

QP8043-ec-10ug 10ug
EUR 240

Recombinant Human Programmed cell death protein 10 Protein, His-SUMO, E.coli-200ug

QP8043-ec-200ug 200ug
EUR 760.8

Recombinant Human Programmed cell death protein 10 Protein, His-SUMO, E.coli-500ug

QP8043-ec-500ug 500ug
EUR 1272

Recombinant Human Programmed cell death protein 10 Protein, His-SUMO, E.coli-50ug

QP8043-ec-50ug 50ug
EUR 315.6

PDCD6 Programmed Cell Death 6 Human Recombinant Protein

PROTO75340 Regular: 10ug
EUR 380.4
Description: PDCD6 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 211 amino acids (1-191a.a.) and having a molecular mass of 24.0 kDa. ;PDCD6 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

PDCD4 Programmed Cell Death 4 Human Recombinant Protein

PROTQ53EL6 Regular: 10ug
EUR 380.4
Description: PDCD4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 469 amino acids and having a molecular mass of 51 kDa.

Recombinant Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1)

4-RPA788Hu01
  • EUR 571.58
  • EUR 276.00
  • EUR 1813.44
  • EUR 684.48
  • EUR 1248.96
  • EUR 458.40
  • EUR 4353.60
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Programmed Cell Death Protein 1 Ligand 1 expressed in: E.coli

Recombinant Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1)

4-RPA788Mu01
  • EUR 603.84
  • EUR 285.60
  • EUR 1934.40
  • EUR 724.80
  • EUR 1329.60
  • EUR 480.00
  • EUR 4656.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Programmed Cell Death Protein 1 Ligand 1 expressed in: E.coli

Recombinant Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1)

4-RPA788Mu02
  • EUR 623.20
  • EUR 290.40
  • EUR 2006.98
  • EUR 748.99
  • EUR 1377.98
  • EUR 492.00
  • EUR 4837.44
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Programmed Cell Death Protein 1 Ligand 1 expressed in: E.coli

Recombinant Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1)

4-RPA788Ra01
  • EUR 625.34
  • EUR 290.40
  • EUR 2015.04
  • EUR 751.68
  • EUR 1383.36
  • EUR 494.40
  • EUR 4857.60
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Programmed Cell Death Protein 1 Ligand 1 expressed in: E.coli

Recombinant Programmed Cell Death Protein 1 Ligand 2 (PDCD1LG2)

4-RPA789Hu01
  • EUR 582.34
  • EUR 279.60
  • EUR 1853.76
  • EUR 697.92
  • EUR 1275.84
  • EUR 465.60
  • EUR 4454.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Programmed Cell Death Protein 1 Ligand 2 expressed in: E.coli

Recombinant Programmed Cell Death Protein 1 Ligand 2 (PDCD1LG2)

4-RPA789Mu01
  • EUR 588.79
  • EUR 280.80
  • EUR 1877.95
  • EUR 705.98
  • EUR 1291.97
  • EUR 469.20
  • EUR 4514.88
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Programmed Cell Death Protein 1 Ligand 2 expressed in: E.coli

Recombinant Programmed Cell Death Protein 1 Ligand 2 (PDCD1LG2)

4-RPA789Ra01
  • EUR 593.09
  • EUR 282.00
  • EUR 1894.08
  • EUR 711.36
  • EUR 1302.72
  • EUR 472.80
  • EUR 4555.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Programmed Cell Death Protein 1 Ligand 2 expressed in: E.coli

Recombinant Human Programmed Cell Death 5

7-05830 5µg Ask for price

Recombinant Human Programmed Cell Death 5

7-05831 20µg Ask for price

Recombinant Human Programmed Cell Death 5

7-05832 1mg Ask for price

Recombinant Human Programmed Cell Death 6

7-05833 2µg Ask for price

Recombinant Human Programmed Cell Death 6

7-05834 10µg Ask for price

Recombinant Human Programmed Cell Death 6

7-05835 1mg Ask for price

Recombinant Human Programmed cell death protein 1, GST, E.coli-1mg

QP6475-ec-1mg 1mg
EUR 1958.4

Recombinant Human Programmed cell death protein 1, GST, E.coli-1mg

QP8484-ec-1mg 1mg
EUR 1958.4

PDCD1Human Programmed Cell Death 1 Human Recombinant Protein

PROTQ15116 Regular: 10ug
EUR 380.4
Description: PDCD1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 392 amino acids (21-170a.a.) and having a molecular mass of 44.0kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).;PDCD1 is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.

Recombinant Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP)

4-RPB247Hu01
  • EUR 550.08
  • EUR 271.20
  • EUR 1732.80
  • EUR 657.60
  • EUR 1195.20
  • EUR 444.00
  • EUR 4152.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Programmed Cell Death Protein 6 Interacting Protein expressed in: E.coli

Recombinant Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP)

4-RPB247Mu01
  • EUR 560.83
  • EUR 273.60
  • EUR 1773.12
  • EUR 671.04
  • EUR 1222.08
  • EUR 451.20
  • EUR 4252.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Programmed Cell Death Protein 6 Interacting Protein expressed in: E.coli

Recombinant Human Programmed cell death protein 1, GST, E.coli-10ug

QP6475-ec-10ug 10ug
EUR 240

Recombinant Human Programmed cell death protein 1, GST, E.coli-50ug

QP6475-ec-50ug 50ug
EUR 315.6