Concanavalin A

concanavalin a

G Protein Pathways, Part B: G Proteins and their Regulators

Cross-Linking Concanavalin A to SPR Chips
Concanavalin A is a tetrameric protein with four identical 28 kDa subunits. 29 The minimal structure exhibiting high affinity binding of carbohydrates is the dimeric form. The dimer form of ConA is an active stable structure, and the tetramer – dimer transition can be promoted by low pH or succinylation of the protein.30 For reasons of economy we use unmodified concanavalin A (Sigma). Because the dissociation of dimer from tetramer leads to a continual decline in the SPR baseline, our coupling procedures first promote the tetramer – dimer dissociation prior to linkage. To accomplish this, ConA is dissolved and stored at 4 ° in 100 mM sodium acetate, pH 4.8, prior to coupling. This also provides for optimal concentration of the ConA dimer by ionic attraction to the unactivated carboxyl groups. For limiting the ConA coupling the pH can be adjusted to 5.4 and still promote the dimer form.

However, extensive washing of the surface to allow complete tetramer dissociation is then necessary. Linkage of the ConA is accomplished by standard NHS / EDC amine coupling16 as described for streptavidin above, but with a modification in the running solution (Solution C, 50 mM MOPS, pH 7.5, 3 mM MgSO4, 150 mM NaCl with 10 μM CaCl2 and 10 μM MnCl2.

concanavalin a
concanavalin a

First, the surface is activated by injection of a mixture of 0.2 M EDC with 50 mM NHS in water at a flow rate of 5 μl / min, exposure time of 5–7 min. This is followed by injection of ConA at 0.1–0.3 mg / ml in 100 mM sodium acetate, pH 4.8, with an exposure time of 5–7 min. Activated carboxyls are then quenched by reaction with 1 M ethanolamine in water with an exposure time of 4 min. These procedures lead to the immobilization predominantly of dimeric ConA.

The immobilized ConA is quite stable, so that rhodopsin coupling does not have to proceed immediately. However, the coupling reactions are sufficiently reproducible and rapid that we find no advantage to prior preparation of ConA-modified surfaces. For experiments defining the independent binding of Gα or Gβγ described below, surfaces modified with 7000–12,000 RU of ConA have been utilized.

Under these conditions, the presence of immobilized tetrameric ConA is a significant factor. When high concentrations of ConA and / or aggressive NHS / EDC activation conditions are used, we routinely wash the ConA surface for 30–60 min with Solution C at a flow rate of 5 μl / min to allow for complete tetramer – dimer dissociation, prior to rhodopsin immobilization.

 

Concanavalin A (ConA)

05-0106-100 100 mg
EUR 91.2

Concanavalin A (ConA)

05-0106-1000 1 g
EUR 238.8

Concanavalin A (ConA)

05-0106-10000 10 g Ask for price

Concanavalin A (ConA)

05-0106-100000 100 g Ask for price

Concanavalin A (ConA)

05-0106-250 250 mg
EUR 105.6

Concanavalin A IV

abx082153-25mg 25 mg
EUR 309.6

Concanavalin A (Con A) Antibody

20-abx101152
  • EUR 326.40
  • EUR 159.60
  • EUR 811.20
  • EUR 410.40
  • EUR 292.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Concanavalin A, CF350 conjugate

29015 5x1mg
EUR 250.8
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF488A conjugate

29016 5x1mg
EUR 250.8
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF594 conjugate

29017 5x1mg
EUR 250.8
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF633 conjugate

29018 5x1mg
EUR 250.8
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF640R conjugate

29019 5x1mg
EUR 250.8
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF680 conjugate

29020 5x1mg
EUR 288
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF770 conjugate

29058 5x1mg
EUR 288
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF405M conjugate

29074 5x1mg
EUR 262.8
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF405S conjugate

29075 5x1mg
EUR 262.8
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF750 conjugate

29080 5x1mg
EUR 288
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A ELISA Kit

20-abx052181
  • EUR 7726.80
  • EUR 4118.40
  • EUR 961.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Concanavalin A ELISA kit

E01C0795-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Concanavalin A ELISA kit

E01C0795-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Concanavalin A ELISA kit

E01C0795-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Concanavalin A ELISA kit

E07C0795-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Concanavalin A ELISA kit

E07C0795-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Concanavalin A ELISA kit

E07C0795-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Concanavalin A ELISA kit

E06C0795-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Concanavalin A ELISA kit

E06C0795-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Concanavalin A ELISA kit

E06C0795-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Concanavalin A ELISA kit

E04C0795-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Concanavalin A ELISA kit

E04C0795-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Concanavalin A ELISA kit

E04C0795-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Concanavalin A ELISA kit

E08C0795-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Concanavalin A ELISA kit

E08C0795-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Concanavalin A ELISA kit

E08C0795-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Concanavalin A ELISA kit

E09C0795-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Concanavalin A ELISA kit

E09C0795-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Concanavalin A ELISA kit

E09C0795-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.