HMGB1 could restrict 1,3-β-glucan induced mice lung inflammation by affecting Beclin1 and Bcl2 interaction and promoting the autophagy of epithelial cells
Fungi had been microorganisms which are ubiquitous in a wide range of environments. Inhalation of fungi-contaminated natural mud led to hypersensitivity pneumonitis and may finally trigger irreversible pulmonary fibrosis. Research confirmed that sustaining the homeostasis of epithelial cells was important for defending the exogenous fungi invasion. HMGB1-dependent autophagy performed a vital function in sustaining cell homeostasis in a number of inflammatory ailments. Nonetheless, the precise function of HMGB1-dependent autophagy in hypersensitivity pneumonitis was unclear.
In our research, mice had been uncovered to 0.Three mg/50 μL 1,3-β-glucan answer by intratracheal instillation to arrange the lung irritation mannequin. To research the function of HMGB1-dependent autophagy in 1,3-β-glucan induced lung irritation, AAV-sh-HMGB1 was intratracheally injected to silence HMGB1 within the lung. Our discovering instructed that silencing HMGB1 might irritate the 1,3-β-glucan induced lung irritation by inhibiting the autophagy of epithelial cells. And ubiquitination of Beclin1 contributed to lowering the interplay of Beclin1 and Bcl2, which could be a key regulatory mechanism of HMGB1 on 1,3-β-glucan induced autophagy.
An Autochthonous Mouse Mannequin of Myd88– and BCL2-Pushed Diffuse Massive B-cell Lymphoma Reveals Actionable Molecular Vulnerabilities
Based mostly on gene expression profiles, diffuse giant B-cell lymphoma (DLBCL) is subdivided into germinal middle B-cell-like (GCB) and activated B-cell-like (ABC) DLBCL. Two of the commonest genomic aberrations in ABC-DLBCL are mutations in MYD88 in addition to BCL2 copy-number features. Right here, we make use of immune phenotyping, RNA sequencing, and whole-exome sequencing to characterize a Myd88– and BCL2-driven mouse mannequin of ABC-DLBCL. We present that this mannequin resembles options of human ABC-DLBCL. We additional exhibit an actionable dependence of our murine ABC-DLBCL mannequin on BCL2. This BCL2 dependence was additionally detectable in human ABC-DLBCL cell traces.
Furthermore, human ABC-DLBCLs displayed elevated PD-L1 expression in contrast with GCB-DLBCL. In vivo experiments in our ABC-DLBCL mannequin confirmed that mixed venetoclax and PD-1 blockade considerably elevated the general survival of lymphoma-bearing animals, indicating that this mix could also be a viable choice for chosen human ABC-DLBCL instances harboring MYD88 and BCL2 aberrations.
SIGNIFICANCE: Oncogenic Myd88 and BCL2 cooperate in murine DLBCL lymphomagenesis. The ensuing lymphomas show morphologic and transcriptomic options harking back to human ABC-DLBCL. Information derived from our Myd88/BCL2-driven autochthonous mannequin exhibit that mixed BCL2 and PD-1 blockade shows substantial preclinical antilymphoma exercise, offering preclinical proof-of-concept knowledge, which pave the best way for scientific translation.This text is highlighted within the In This Problem function, p. 1.
Transcript-Degree Dysregulation of BCL2 Household Genes in Acute Myeloblastic Leukemia
The expression of apoptosis-related BCL2 household genes, fine-tuned in regular cells, is dysregulated in lots of neoplasms. In acute myeloid leukemia (AML), this downside has not been studied comprehensively. To handle this concern, RNA-seq knowledge had been used to research the expression of 26 BCL2 members of the family in 27 AML FAB M1 and M2 sufferers, divided into subgroups in a different way responding to chemotherapy.
A correlation evaluation, evaluation of variance, and Kaplan-Meier evaluation had been utilized to affiliate the expression of explicit genes with different gene expression, scientific options, and the presence of mutations detected by exome sequencing. The expression of BCL2 household genes was dysregulated in AML, as in comparison with wholesome controls. An upregulation of anti-apoptotic and downregulation of pro-apoptotic genes was noticed, although solely a lower in BMF, BNIP1, and HRK was statistically vital.
In a bunch of sufferers proof against chemotherapy, overexpression of BCL2L1 was manifested. In settlement with the literature knowledge, our outcomes reveal that BCL2L1 is without doubt one of the key gamers in apoptosis regulation in various kinds of tumors. An exome sequencing knowledge evaluation signifies that BCL2 household genes are usually not mutated in AML, however their expression is correlated with the mutational standing of different genes, together with these recurrently mutated in AML and splicing-related. Excessive ranges of some BCL2 members of the family, specifically BIK and BCL2L13, had been related to poor consequence.
Expression and Scientific Significance of BCL2 Interacting Protein Three Like in A number of Myeloma
A number of myeloma (MM) is without doubt one of the major blood issues threatening human well being as we speak. This research aimed to look at the expression of BCL-2/adenovirus E1B 19 kDa-interacting protein 3-like (BNIP3L) in sufferers with MM and discover its mechanisms in silico. Bone marrow samples (n = 36 from sufferers with MM and n = 12 from wholesome donors) had been used to conduct BNIP3L expression evaluation utilizing immunohistochemistry.
Microarray or RNA sequencing knowledge from the Sequence Learn Archive, Gene Expression Omnibus, and ArrayExpress databases had been used to appraise BNIP3L expression and its prognostic function in sufferers with MM. The co-expressed genes of BNIP3L had been recognized for enrichment and protein-protein interplay (PPI) analyses to find out the related signaling pathways. Immunohistochemistry indicated that BNIP3L expression in bone marrow of sufferers with MM was considerably decrease than that in bone marrow of wholesome donors. BNIP3L mRNA expression was additionally considerably decrease in sufferers with MM than in wholesome donors. The general customary imply distinction (SMD) for downregulation of BNIP3L was -0.62 [-1.17, -0.06], and the world underneath the curve was 0.81 [0.78, 0.85] based mostly on a complete of 694 MM instances.
The general survival evaluation demonstrated that BNIP3L ranges might act as an unbiased protecting indicator of MM affected person survival (HR = 0.79). Furthermore, 261 co-expressed genes of BNIP3L had been confirmed and located to be primarily concerned within the adipocytokine signaling pathway. We preliminarily proved that downregulation of BNIP3L could play an essential function within the prevalence and growth of MM, and the marketing most cancers capability could also be associated to the pathway of adipocytokine signaling pathway.
Key phrases: BNIP3L; immunohistochemistry; a number of myeloma; silico; suppressor gene.
 Recombinant Human Hydroxymethylglutaryl-CoA lyase, GST, E.coli-10ug |
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| QP6168-ec-10ug | EnQuireBio | 10ug | EUR 240 |
 Recombinant Human Hydroxymethylglutaryl-CoA lyase, GST, E.coli-200ug |
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| QP6168-ec-200ug | EnQuireBio | 200ug | EUR 760.8 |
 Recombinant Human Hydroxymethylglutaryl-CoA lyase, GST, E.coli-500ug |
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| QP6168-ec-500ug | EnQuireBio | 500ug | EUR 1272 |
 Recombinant Human Hydroxymethylglutaryl-CoA lyase, GST, E.coli-50ug |
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| QP6168-ec-50ug | EnQuireBio | 50ug | EUR 315.6 |
) Argininosuccinate Lyase (Recombinant) |
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| 20-abx073248 | Abbexa |
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) Recombinant Sphingosine 1 Phosphate Lyase 1 (SGPL1) |
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| 4-RPE691Hu01 | Cloud-Clone |
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Description: Recombinant Human Sphingosine 1 Phosphate Lyase 1 expressed in: E.coli |
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Recombinant Sphingosine 1 Phosphate Lyase 1 (SGPL1) |
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| 4-RPE691Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Sphingosine 1 Phosphate Lyase 1 expressed in: E.coli |
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 Cystathionine β-lyase, Recombinant |
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| NATE-1146 | Creative Enzymes | 100mg | EUR 712.8 |
 Recombinant human Selenocysteine lyase |
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| P2183 | FN Test | 100ug | Ask for price |
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Description: Recombinant protein for human Selenocysteine lyase |
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 Selenocysteine Lyase Recombinant Protein |
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| 92-018 | ProSci | 0.05 mg | EUR 852.9 |
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Description: Selenocysteine Lyase belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Selenocysteine Lyase exists as a homodimer in the cytosol. In the brain, Selenocysteine Lyase is as an enzyme that putatively salvages Sec and recycles the selenium for selenoprotein translation. Selenocysteine Lyase catalyzes the decomposition of L-selenocysteine to L-alanine and elemental selenium. Selenocysteine Lyase can be up-regulated In acute glomerulonephritis, it can also be regulated by JUN/AP-1. |
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 HMGCL 3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase Human Recombinant Protein |
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| PROTP35914 | BosterBio | Regular: 20ug | EUR 380.4 |
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Description: HMGCL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 323 amino acids (28-325) and having a molecular mass of 34.2kDa.;HMGCL is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
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) Recombinant Cystathionine Gamma Lyase (CSE) |
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| 4-RPB538Hu01 | Cloud-Clone |
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Description: Recombinant Human Cystathionine Gamma Lyase expressed in: E.coli |
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Recombinant Cystathionine Gamma Lyase (CSE) |
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| 4-RPB538Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Cystathionine Gamma Lyase expressed in: E.coli |
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Recombinant Cystathionine Gamma Lyase (CSE) |
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| 4-RPB538Ra01 | Cloud-Clone |
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Description: Recombinant Rat Cystathionine Gamma Lyase expressed in: E.coli |
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 Recombinant E.Coli N-Acetylneuraminate Lyase |
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| 7-03193 | CHI Scientific | 5µg | Ask for price |
Recombinant E.Coli N-Acetylneuraminate Lyase |
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| 7-03194 | CHI Scientific | 20µg | Ask for price |
Recombinant E.Coli N-Acetylneuraminate Lyase |
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| 7-03195 | CHI Scientific | 1mg | Ask for price |
) E.Coli N-Acetylneuraminate Lyase (Recombinant) |
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| 20-abx073216 | Abbexa |
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, Active, Recombinant) Homocysteine ?,?-Lyase (rHCYase), Active, Recombinant |
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| P1117-200 | Biovision | each | EUR 510 |
) N-acetylneuraminate Pyruvate Lyase (Recombinant) |
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| 20-abx073214 | Abbexa |
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 Recombinant Human Cystathionine γ-Lyase/CTH |
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| CF60-10ug | Novoprotein | 10ug | EUR 175.2 |
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Description: Supplied as a 0.2 μm filtered solution of 20mM PB,150mM NaCl,20% Glycerol,pH7.4. |
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Recombinant Human Cystathionine γ-Lyase/CTH |
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| CF60-1mg | Novoprotein | 1mg | EUR 2739.6 |
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Description: Supplied as a 0.2 μm filtered solution of 20mM PB,150mM NaCl,20% Glycerol,pH7.4. |
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Recombinant Human Cystathionine γ-Lyase/CTH |
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| CF60-500ug | Novoprotein | 500ug | EUR 1935.6 |
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Description: Supplied as a 0.2 μm filtered solution of 20mM PB,150mM NaCl,20% Glycerol,pH7.4. |
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Recombinant Human Cystathionine γ-Lyase/CTH |
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| CF60-50ug | Novoprotein | 50ug | EUR 406.8 |
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Description: Supplied as a 0.2 μm filtered solution of 20mM PB,150mM NaCl,20% Glycerol,pH7.4. |
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 ATP citrate lyase recombinant monoclonal antibody |
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| A5181 | Bimake | 100ul X 3 | EUR 714 |
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Description: A recombinant monoclonal antibody from rabbit against human ATP citrate lyase for WB,ELISA |
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 HMGCL Human, 3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase Human Recombinant Protein, Sf9 |
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| PROTP35914-1 | BosterBio | Regular: 10ug | EUR 380.4 |
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Description: HMGCL Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 305 amino acids (28-325 a.a.) and having a molecular mass of 32.5kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). HMGCL is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques. |
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 L-Methionine ?-Lyase, Pseudomonas putida recombinant |
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| 7848-100 | Biovision | each | EUR 189.6 |
L-Methionine ?-Lyase, Pseudomonas putida recombinant |
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| 7848-500 | Biovision | each | EUR 464.4 |
 ASL Argininosuccinate Lyase Human Recombinant Protein |
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| PROTP04424 | BosterBio | Regular: 20ug | EUR 380.4 |
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Description: ASL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 484 amino acids (1-464) and having a molecular mass of 53.8kDa.;ASL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
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 ADSL Adenylosuccinate Lyase Human Recombinant Protein |
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| PROTP30566 | BosterBio | Regular: 20ug | EUR 380.4 |
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Description: ADSL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 520 amino acids (1-484) and having a molecular mass of 59kDa.;ADSL is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
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 Recombinant Human N-acetylneuraminate Pyruvate Lyase |
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| 7-03232 | CHI Scientific | 5µg | Ask for price |
Recombinant Human N-acetylneuraminate Pyruvate Lyase |
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| 7-03233 | CHI Scientific | 20µg | Ask for price |
Recombinant Human N-acetylneuraminate Pyruvate Lyase |
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| 7-03234 | CHI Scientific | 1mg | Ask for price |
) Recombinant Human Selenocysteine Lyase/SCLY (N-6His) |
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| CE52-10ug | Novoprotein | 10ug | EUR 242.4 |
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Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0. |
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Recombinant Human Selenocysteine Lyase/SCLY (N-6His) |
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| CE52-1mg | Novoprotein | 1mg | EUR 2739.6 |
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Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0. |
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Recombinant Human Selenocysteine Lyase/SCLY (N-6His) |
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| CE52-500ug | Novoprotein | 500ug | EUR 1935.6 |
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Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0. |
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Recombinant Human Selenocysteine Lyase/SCLY (N-6His) |
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| CE52-50ug | Novoprotein | 50ug | EUR 595.2 |
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Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0. |
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 Hyaluronate Lyase from Streptococcus pyogenes, recombinant |
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| NATE-0346 | Creative Enzymes | 10mg | EUR 440.4 |
) Recombinant 2-Hydroxyacyl Coenzyme A Lyase 1 (HACL1) |
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| 4-RPG361Hu01 | Cloud-Clone |
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Description: Recombinant Human 2-Hydroxyacyl Coenzyme A Lyase 1 expressed in: E.coli |
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 NANA N-Acetylneuraminate Lyase E.Coli Recombinant Protein |
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| PROTP0A6L4 | BosterBio | Regular: 20ug | EUR 380.4 |
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Description: NANA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 317 amino acids (1-297 a.a.) and having a molecular mass of 34.7kDa.;NANA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
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) Recombinant Cysteine Conjugate Beta Lyase, Cytoplasmic (CCbL1) |
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| 4-RPD863Hu01 | Cloud-Clone |
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Description: Recombinant Human Cysteine Conjugate Beta Lyase, Cytoplasmic expressed in: E.coli |
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 L-Methionine γ-Lyase from Pseudomonas putida, Recombinant |
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| NATE-1655 | Creative Enzymes | 50u | EUR 712.8 |
 NPL N-acetylneuraminate Pyruvate Lyase Human Recombinant Protein |
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| PROTQ9BXD5 | BosterBio | Regular: 20ug | EUR 380.4 |
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Description: NPL produced in E.Coli is a single, non-glycosylated polypeptide chain containing 340 amino acids (1-320 a.a.) and having a molecular mass of 37.3kDa.;NPL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
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 Recombinant Human N-glycosylase/ DNA lyase Protein, His, E.coli-1mg |
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| QP6448-ec-1mg | EnQuireBio | 1mg | EUR 1958.4 |
 Recombinant E.coli S-ribosylhomocysteine lyase Protein, His, E.coli-1mg |
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| QP7508-ec-1mg | EnQuireBio | 1mg | EUR 2763.6 |
 Recombinant Human N-glycosylase/ DNA lyase Protein, His, E.coli-10ug |
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| QP6448-ec-10ug | EnQuireBio | 10ug | EUR 240 |
 Recombinant Human N-glycosylase/ DNA lyase Protein, His, E.coli-50ug |
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| QP6448-ec-50ug | EnQuireBio | 50ug | EUR 315.6 |
 Recombinant Human N-glycosylase/ DNA lyase Protein, His, Yeast-1mg |
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| QP6448-ye-1mg | EnQuireBio | 1mg | EUR 2763.6 |
 Recombinant Human N-glycosylase/ DNA lyase Protein, His, E.coli-100ug |
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| QP6448-ec-100ug | EnQuireBio | 100ug | EUR 489.6 |
 Recombinant Human N-glycosylase/ DNA lyase Protein, His, E.coli-200ug |
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| QP6448-ec-200ug | EnQuireBio | 200ug | EUR 760.8 |
 Recombinant Human N-glycosylase/ DNA lyase Protein, His, E.coli-500ug |
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| QP6448-ec-500ug | EnQuireBio | 500ug | EUR 1272 |
 Recombinant Human N-glycosylase/ DNA lyase Protein, His, Yeast-10ug |
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| QP6448-ye-10ug | EnQuireBio | 10ug | EUR 326.4 |
 Recombinant Human N-glycosylase/ DNA lyase Protein, His, Yeast-50ug |
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| QP6448-ye-50ug | EnQuireBio | 50ug | EUR 424.8 |
 Recombinant E.coli S-ribosylhomocysteine lyase Protein, His, E.coli-100ug |
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| QP7508-ec-100ug | EnQuireBio | 100ug | EUR 848.4 |
 Recombinant E.coli S-ribosylhomocysteine lyase Protein, His, E.coli-10ug |
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| QP7508-ec-10ug | EnQuireBio | 10ug | EUR 391.2 |
 Recombinant E.coli S-ribosylhomocysteine lyase Protein, His, E.coli-200ug |
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| QP7508-ec-200ug | EnQuireBio | 200ug | EUR 1338 |
 Recombinant E.coli S-ribosylhomocysteine lyase Protein, His, E.coli-500ug |
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| QP7508-ec-500ug | EnQuireBio | 500ug | EUR 1816.8 |
 Recombinant E.coli S-ribosylhomocysteine lyase Protein, His, E.coli-50ug |
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| QP7508-ec-50ug | EnQuireBio | 50ug | EUR 522 |
 Recombinant Human N-glycosylase/ DNA lyase Protein, His, Yeast-100ug |
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| QP6448-ye-100ug | EnQuireBio | 100ug | EUR 685.2 |
 Recombinant Human N-glycosylase/ DNA lyase Protein, His, Yeast-200ug |
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| QP6448-ye-200ug | EnQuireBio | 200ug | EUR 1077.6 |
 Recombinant Human N-glycosylase/ DNA lyase Protein, His, Yeast-500ug |
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| QP6448-ye-500ug | EnQuireBio | 500ug | EUR 1806 |
 Recombinant Human Cytochrome c-type heme lyase Protein, GST, E.coli-1mg |
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| QP6149-ec-1mg | EnQuireBio | 1mg | EUR 2763.6 |
 anti-2 Hydroxy phytanoyl CoA lyase |
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| YF-PA18196 | Abfrontier | 50 ul | EUR 435.6 |
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Description: Mouse polyclonal to 2 Hydroxy phytanoyl CoA lyase |
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anti-2 Hydroxy phytanoyl CoA lyase |
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| YF-PA18197 | Abfrontier | 50 ug | EUR 435.6 |
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Description: Mouse polyclonal to 2 Hydroxy phytanoyl CoA lyase |
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 CCBL1 Cysteine Conjugate-Beta Lyase Cytoplasmic Human Recombinant Protein |
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| PROTQ16773 | BosterBio | Regular: 20ug | EUR 380.4 |
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Description: CCBL1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 445 amino acids (1-422 a.a) and having a molecular mass of 50.3kDa. CCBL1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
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) Recombinant 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase (HMGCL) |
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| 4-RPD685Hu01 | Cloud-Clone |
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Description: Recombinant Human 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase expressed in: E.coli |
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Recombinant 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase (HMGCL) |
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| 4-RPD685Mu01 | Cloud-Clone |
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Description: Recombinant Mouse 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase expressed in: E.coli |
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 Recombinant Human Cytochrome c-type heme lyase Protein, GST, E.coli-100ug |
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| QP6149-ec-100ug | EnQuireBio | 100ug | EUR 685.2 |
 Recombinant Human Cytochrome c-type heme lyase Protein, GST, E.coli-10ug |
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| QP6149-ec-10ug | EnQuireBio | 10ug | EUR 326.4 |
 Recombinant Human Cytochrome c-type heme lyase Protein, GST, E.coli-200ug |
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| QP6149-ec-200ug | EnQuireBio | 200ug | EUR 1077.6 |
 Recombinant Human Cytochrome c-type heme lyase Protein, GST, E.coli-500ug |
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| QP6149-ec-500ug | EnQuireBio | 500ug | EUR 1816.8 |
 Recombinant Human Cytochrome c-type heme lyase Protein, GST, E.coli-50ug |
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| QP6149-ec-50ug | EnQuireBio | 50ug | EUR 434.4 |
 Recombinant Human ACLY/ acly/ ATP citrate lyase Protein, His-SUMO, E.coli-10ug |
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| QP5607-ec-10ug | EnQuireBio | 10ug | EUR 326.4 |
 Recombinant Human ACLY/ acly/ ATP citrate lyase Protein, His-SUMO, E.coli-1mg |
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| QP5607-ec-1mg | EnQuireBio | 1mg | EUR 2763.6 |
 Recombinant Human ACLY/ acly/ ATP citrate lyase Protein, His-SUMO, E.coli-50ug |
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| QP5607-ec-50ug | EnQuireBio | 50ug | EUR 434.4 |
 Sphaerotilus natans RT PCR kit |
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| RTq-A576-100D | Bioingentech | 100T | EUR 860.4 |
Sphaerotilus natans RT PCR kit |
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| RTq-A576-150D | Bioingentech | 150T | EUR 969.6 |
