Inhibitory effect of organic acid salts on spoilage flora in culture medium and cured cooked meat products under commercial manufacturing conditions.
Lactobacillus curvatus, remoted from a spoiled vacuum-packaged ‘pariza’ kind meat product, was used to inoculate modified MRS broth containing sodium lactate, sodium acetate and potassium sorbate in several concentrations, alone or in inter se combos. Two industrial preparations (MIX 1 and MIX 2) have been additionally used containing combos of the above antimicrobials.
Outcomes from the preservatives addition to the tradition medium confirmed highest antimicrobial exercise within the case of the sodium lactate (2%, 3% or 4%), sodium acetate (0.5%) and potassium sorbate (0.15%) mixture. Outcomes from the preservatives addition to 2 forms of thermally processed meats confirmed that sodium lactate and the mixture of sodium lactate, sodium acetate and potassium sorbate have been the simplest; extending the merchandise shelf life an extra 10 days. Lastly, MIX 1 and MIX 2 suppressed the lactic acid micro organism (LAB) progress within the tradition medium however not within the ultimate product.
AOAC technique 966.04: preliminary analysis of cooked meat medium with manganese sulfate for the cultivation of Clostridium sporogenes: precollaborative examine.
AOAC Methodology 966.04, the Sporicidal Exercise of Disinfectants Take a look at, is a carrier-based take a look at that gives a qualitative measure of product efficacy in opposition to spores of Bacillus subtilis and Clostridium sporogenes. For regulatory functions, Methodology 966.04 is accepted by the U.S. Environmental Safety Company (EPA) and the U.S. Meals and Drug Administration (FDA) for the era of product efficiency information for sporicides and sterilants. On this examine, we report on findings related to proposed enhancements (modifications) to the Clostridium element of the strategy.
Egg meat medium (EMM), the tradition medium for C. sporogenes at the moment specified within the technique, is not commercially accessible and discovering an appropriate substitute is important. As well as, using a nonstandardized extract of uncooked soil as an modification to EMM, as stipulated within the present technique, could end in a extremely variable spore suspension.
The first focus of this examine was to seek out replacements for EMM and soil extract. A provider rely process, the institution of goal provider counts (spores/provider), and a neutralization affirmation process have been additionally evaluated. The examine was restricted to liquid merchandise examined in opposition to Clostridium on a tough floor provider (porcelain penicylinder). Spore suspensions of C. sporogenes have been generated utilizing: (1) EMM with soil extract (EMM/SE), (2) cooked meat medium with soil extract (CMM/SE), and (3) cooked meat medium with 5 microg/mL manganese sulfate (CMM/MnSO4).
The titer of the spore suspension, provider counts, resistance to hydrochloric acid (HCI), and efficacy in opposition to Three liquid sporicidal brokers have been used to guage the potential of CMM and MnSO4 as replacements. The examine was carried out by the EPA Workplace of Pesticide Applications Microbiology Laboratory, Fort Meade, MD. Use of CMM/SE and CMM/MnSO4 resulted in comparable outcomes for titer of spore suspensions (roughly 10(8) spores/mL) and provider counts (roughly Three x 10(6) spores/provider). The provider counts for the EMM/SE have been roughly 1 log decrease than CMM-based remedies; nevertheless, no try was made to dilute the CMM spore suspensions previous to provider inoculation to scale back the provider counts for CMM. Resistance of spores to 2.5 M HCI was acceptable throughout the three media varieties.
Remedies for comparative efficacy testing have been designed to supply a variety of sporicidal exercise, i.e., excessive and low efficacy remedies. Sodium hypochlorite (bleach), hydrogen peroxide/peracetic acid, and glutaraldehyde have been used as take a look at chemical compounds. The variety of carriers leading to progress (constructive) for the low remedies for all Three chemical compounds ranged from 9 to 59 out of 60 throughout the three media types–EMM exhibited fewer positives general.
The excessive efficacy remedies for sodium hypochlorite and hydrogen peroxide/peracetic acid yielded a variety of Zero to 2 positives out of 60 throughout the three media. Nonetheless, the excessive glutaraldehyde therapy generated 3, 20, and 20 positives out of 60 for the EMM/SE, CMM/SE, and CMM/MnSO4, respectively. The decrease variety of constructive carriers for EMM/SE could also be because of the decreased provider counts. CMM, both with SE or MnSO4, seems to be an appropriate substitute for EMM/SE.
On the premise of the outcomes of this examine, the Examine Director recommends that CMM/MnSO4 and the spore enumeration goal provider rely and neutralization procedures be thought of for collaborative examine to formally modify the Clostridium x porcelain element of Methodology 966.04.
Synthetic neural network-based predictive mannequin for bacterial progress in a simulated medium of modified-atmosphere-packed cooked meat merchandise.
The information of Devilieghere et al. (Int. J. Meals Microbiol. 1999, 46, 57–70) on bacterial progress in a simulated medium of modified-atmosphere-packed cooked meat merchandise was processed for estimating most particular progress price mu(max) and lag part lambda of Lactobacillus sake utilizing synthetic neural networks-based mannequin (ANNM) computation.
Cooked Meat-BHI Overlay |
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| MED1036 | Scientific Laboratory Supplies | PK50 |
Cooked Meat-FAB Overlay |
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| MED1136 | Scientific Laboratory Supplies | PK50 |
Cooked Meat-FAB Overlay |
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| MED1684 | Scientific Laboratory Supplies | PK50 |
Hanna HI-151-3 Checktemp 4 Folding Thermometer for Cooked Meat - Yellow |
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| THE4564 | Scientific Laboratory Supplies | EACH |
Meat Extract |
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| MED1512 | Scientific Laboratory Supplies | EACH |
Meat Peptone |
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| MED1514 | Scientific Laboratory Supplies | EACH |
CASEIN MEAT MIXTURE |
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| C03-106-10kg | Alphabiosciences | 10 kg |
CASEIN MEAT MIXTURE |
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| C03-106-2kg | Alphabiosciences | 2kg |
CASEIN MEAT MIXTURE |
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| C03-106-500g | Alphabiosciences | 500 g |
Meat pH electrode |
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| PHE0208 | Scientific Laboratory Supplies | EACH |
Meat pH meter |
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| PHM0334 | Scientific Laboratory Supplies | EACH |
Peptone A FROM MEAT |
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| G213 | Bio Basic | 100g |
Protect Select Meat Free |
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| SWA1192 | Scientific Laboratory Supplies | PK25 |
pH Tester for Meat Testing |
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| PHM2910 | Scientific Laboratory Supplies | EACH |
1-hour Dog meat adulteration ELISA test (for beef or pig meat; 96 tests) |
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| DMT-96 | Alpha Diagnostics | 1 kit |
1-hour Rat meat adulteration ELISA test (for beef or pig meat; 96 tests) |
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| RMT-96 | Alpha Diagnostics | 1 kit |
Meat pH electrode for HI-2002 |
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| PHE0206 | Scientific Laboratory Supplies | EACH |
1-hour Beef/Cow meat adulteration ELISA test (for beef or pig meat; 96 tests) |
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| BMT-96 | Alpha Diagnostics | 1 kit |
1-hour Horse/Donkey meat adulteration ELISA test (for beef or pig meat; 96 tests) |
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| HMT-96 | Alpha Diagnostics | 1 Kit |
1-hour Pig meat adulteration ELISA test (for beef or pig meat; 96 tests), Quantitative |
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| PMT-96 | Alpha Diagnostics | 1 kit |
Meat sample spatulas (individually wrapped) 100/pk |
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| MSSW-100 | Alpha Diagnostics | 1 pk |
Meat sample spatulas (individually wrapped) 1000/pk |
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| MSSW-1000 | Alpha Diagnostics | 1 pk |
A Medium |
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| DJ1018 | Bio Basic | 100g |
DC MEDIUM |
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| D04-117-10kg | Alphabiosciences | 10 kg |
DC MEDIUM |
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| D04-117-2kg | Alphabiosciences | 2kg |
DC MEDIUM |
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| D04-117-500g | Alphabiosciences | 500 g |
Advanced Medium |
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| C0003-04 | Addexbio | RT 500 mL Bottle |
Medium 199 |
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| C0012-01 | Addexbio | RT 500 mL Bottle |
COLIFORM MEDIUM |
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| C03-127-10kg | Alphabiosciences | 10 kg |
COLIFORM MEDIUM |
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| C03-127-2kg | Alphabiosciences | 2kg |
The comparability between ANNM and response floor methodology (RSM) mannequin confirmed that the accuracy of ANNM prediction was greater than that of RSM. Two-dimensional and three-dimensional plots of the response surfaces revealed that the relationships of water exercise a(w), temperature T, and dissolved CO(2) focus with mu(max) and lambda have been sophisticated, not simply linear or second-order relations. Moreover, it was doable to compute the sensitivity of the mannequin outputs in opposition to every enter parameter through the use of ANNM. The outcomes confirmed that mu(max) was most delicate to a(w), T, and dissolved CO(2) on this order; whereas lambda was delicate to T essentially the most, adopted by a(w), and dissolved CO(2) concentrations.