Oxidized fish oils increased lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the CREB1-Bcl2-Beclin1 pathway in the liver tissues and hepatocytes of yellow catfish
At current, the dangerous results and related mechanism of oxidized fish oils on fish and fish cells stay unknown. Our examine discovered that oxidized fish oils elevated lipogenesis, and diminished lipolysis, activated oxidative stress by reducing glutathione peroxidase (GPX) exercise, rising malondialdhyde (MDA) content material and damaging mitochondrial construction, and activated autophagy within the liver of yellow catfish; oxidized eicosapentaenoic acid (oxEPA) induced oxidative stress in yellow catfish hepatocytes. Oxidative stress, mitochondrial dysfunction and lipophagy mediated oxEPA induced-variations in lipid metabolism. Our additional investigation indicated that oxEPA-activated lipophagy was through inhibiting the DNA binding capability of the cAMP-response factor binding protein (CREB)-1 to the area of Bcl-2 promoter, which in flip suppressed the binding exercise of Bcl-2 to Beclin1 and promoted autophagosome formation. For the primary time, our examine elucidated the mechanisms of oxidized fish oils-induced lipid deposition by the oxidative stress, mitochondrial dysfunction and CREB1-Bcl-2-Beclin1 pathway in fish.
Acute toxicity evaluation of an inhibitor of BCL2, Disarib, in rats
Apoptosis or programmed cell loss of life is a extremely regulated course of, which eliminates undesirable and broken cells. Inhibition of apoptosis is a trademark of most cancers cells. BCL2 household proteins are recognized to play an important function within the regulation of apoptosis. Overexpression of BCL2, an antiapoptotic protein, offers the benefit of extended survival to most cancers cells. Over time, a number of BCL2 inhibitors have been investigated extensively for his or her anticancer potential. Nevertheless, most of them have been abolished earlier than scientific use because of their negative effects. Beforehand, we had recognized and characterised a novel BCL2 inhibitor, Disarib, with the potential to remove tumor cells in a BCL2 particular method resulting in discount in tumor burden in a number of mouse fashions.
Notably, a head-to-head comparability of Disarib to ABT199, the one FDA authorised BCL2 inhibitor revealed that Disarib is as potent as ABT199. Current research utilizing mice revealed that Disarib didn’t invoke important negative effects in mice. Within the current examine, we now have investigated the acute toxicity of Disarib in Wistar rats. The bioavailability research following publicity of Disarib in Wistar rats revealed its most availability in serum at 24 h following oral administration. Acute toxicity evaluation revealed that even a dose as excessive as 2000 mg/kg of Disarib didn’t trigger important toxicity in rats.
There was no important variation in blood parameters or kidney and liver capabilities following administration of Disarib. Histological evaluation of various tissues from Disarib handled teams revealed normal structure with no observable mobile injury. Importantly, publicity to Diasrib didn’t lead to genotoxicity as decided by micronucleus assay. Additional, solubility assays revealed that moreover DMSO, Disarib can also be soluble in alcohol. Whereas the excessive acidic situation can improve the solubility of Disarib, even a decrease proportion of alcohol with acidic situations can enhance its solubility. Thus, the toxicological profile within the present examine revealed no important negative effects when Disarib was administered orally to rats.
A brand new pathological classification of intrahepatic cholangiocarcinoma in keeping with protein expression of SSTR2 and Bcl2
Background: No common classification methodology for intrahepatic cholangiocarcinoma (IHCC) has been reported primarily based on the embryological origin of biliary epithelial cells. The intention of this examine was to categorise IHCC in keeping with protein expression ranges of somatostatin receptor 2 (SSTR2) and b-cell leukemia/lymphoma 2 (Bcl2) and to elucidate the clinicopathological options of every group.
Strategies: Fifty-two IHCC sufferers who underwent hepatic resection have been enrolled on this examine. Protein expression ranges of SSTR2 and Bcl2 have been examined utilizing immunohistochemistry. Clinicopathological elements have been in contrast between the three teams and prognostic elements have been investigated.
Outcomes: The sufferers have been divided into three teams: SSTR2 constructive and Bcl2 adverse (p-Group H, n = 21), SSTR2 adverse and Bcl2 constructive (p-Group P, n = 14), and the indeterminate group (p-Group U, n = 17) for circumstances the place SSTR2 and Bcl2 have been each constructive or each adverse. All p-Group P circumstances displayed curability A or B. The 5-year survival charges of p-Group H and U sufferers have been worse than these in p-Group P. p-Group H had greater T-factor, scientific stage, and incidence of periductal infiltration than p-Group P.
Conclusions: This methodology may very well be used to categorise IHCC into peripheral and perihilar kind by embryological expression patterns of SSTR2 and Bcl2.
Key phrases: Bcl2; Clinicopathological traits; Immunohistochemistry; Prognosis; SSTR2.
Down regulation of lactate dehydrogenase initiates apoptosis in HeLa and MCF-7 most cancers cells by way of elevated voltage-dependent anion channel protein and inhibition of BCL2
Malignant cells generally use cardio glycolysis for ATP manufacturing; this is called the Warburg impact, the place pyruvate is transformed to lactate, by enzyme lactate dehydrogenase A (LDH-A). On this examine, we now have investigated the impact of inhibition of LDH-A on cells viability and figuring out the mechanism of cell loss of life in HeLa and MCF-7 most cancers cells. Human cervical most cancers HeLa cell line and breast most cancers MCF-7 cell line have been used to research the impact of inhibition of LDH-A by sodium oxamate on cell survival and proliferation utilizing western blot, spectrophotometry, and immunofluorescent examine.
There was important discount in LDH-A (P < 0.001) and cell viability (P < 0.001) in a dose-dependent mode in each HeLa and MCF-7 SO-treated most cancers cells. The voltage-dependent anion channel (VDAC) protein was considerably elevated (P < 0.001) in affiliation with decreased LDH-A. The proapoptotic proteins; cytochrome C (P < 0.001), BAX (P < 0.001), cleaved caspase-3 (P < 0.001), cleaved caspase-8 (P < 0.001), and cleaved caspase-9 (P < 0.001) have been considerably elevated in affiliation with decreased LDH-A. Whereas, the anti-apoptotic protein Bcl2 was considerably decreased (P < 0.001) in affiliation with decreased LDH-A. We conclude that Inhibition of LDH-A can lower cells viability by way of activation of intrinsic apoptotic pathway through elevated VDAC protein and inhibition of Bcl2 in addition to activation of the extrinsic apoptotic pathway by way of activation of caspase-8.
 Antibody) Escherichia Coli Protein (ECP) Antibody |
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| 20-abx218321 | Abbexa |
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) Escherichia coli Protein yebF (yebF) |
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| 1-CSB-EP329240ENV | Cusabio |
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Description: Recombinant Escherichia coli Protein yebF(yebF) expressed in E.coli |
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 Escherichia coli Protein ELISA Kit |
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| ELA-E1655o | Lifescience Market | 96 Tests | EUR 1113.6 |
 Antibody (Biotin)) Escherichia coli (E. coli) Antibody (Biotin) |
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| abx415713-1ml | Abbexa | 1 ml | EUR 861.6 |
Escherichia coli (E. coli) Antibody (Biotin) |
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| abx411321-1ml | Abbexa | 1 ml | EUR 710.4 |
 Antibody (FITC)) Escherichia coli (E. coli) Antibody (FITC) |
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| abx411322-1ml | Abbexa | 1 ml | EUR 610.8 |
) Escherichia coli Agmatinase (speB) |
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| 1-CSB-EP001447ENM | Cusabio |
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Description: Recombinant Escherichia coli Agmatinase(speB) expressed in E.coli |
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) Escherichia coli Ribokinase (rbsK) |
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| 1-CSB-EP019397ENV | Cusabio |
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Description: Recombinant Escherichia coli Ribokinase(rbsK) expressed in E.coli |
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) Escherichia coli Flagellin (fliC) |
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| 1-CSB-EP300968ENV | Cusabio |
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Description: Recombinant Escherichia coli Flagellin(fliC) expressed in E.coli |
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Escherichia coli Flagellin (fliC) |
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| 1-CSB-EP300968ENVe1 | Cusabio |
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Description: Recombinant Escherichia coli Flagellin(fliC) expressed in E.coli |
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) Escherichia coli Exopolyphosphatase (ppx) |
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| 1-CSB-EP365319ENV | Cusabio |
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Description: Recombinant Escherichia coli Exopolyphosphatase(ppx) expressed in E.coli |
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 Escherichia coli PCR kit |
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| PCR-VHA207-48D | Bioingentech | 50T | EUR 543.6 |
Escherichia coli PCR kit |
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| PCR-VHA207-96D | Bioingentech | 100T | EUR 686.4 |
 Top10F Escherichia coli Strains |
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| S0024 | Lifescience Market | 100 ul | EUR 438 |
 TG1 Escherichia coli Strains |
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| S0026 | Lifescience Market | 100 ul | EUR 438 |
 HB101 Escherichia coli Strains |
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| S0031 | Lifescience Market | 100 ul | EUR 438 |
 DH10B Escherichia coli Strains |
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| S0032 | Lifescience Market | 100 ul | EUR 438 |
 DH10Bac Escherichia coli Strains |
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| S0033 | Lifescience Market | 100 ul | EUR 438 |
 Stbl2 Escherichia coli Strains |
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| S0034 | Lifescience Market | 100 ul | EUR 438 |
 Stbl3 Escherichia coli Strains |
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| S0035 | Lifescience Market | 100 ul | EUR 438 |
 BJ5183 Escherichia coli Strains |
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| S0036 | Lifescience Market | 100 ul | EUR 438 |
 JM110 Escherichia coli Strains |
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| S0037 | Lifescience Market | 100 ul | EUR 438 |
 ER2566 Escherichia coli Strains |
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| S0039 | Lifescience Market | 100 ul | EUR 438 |
 SURE Escherichia coli Strains |
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| S0041 | Lifescience Market | 100 ul | EUR 438 |
 DB3.1 Escherichia coli Strains |
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| S0043 | Lifescience Market | 100 ul | EUR 438 |
 TransB Escherichia coli Strains |
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| S0045 | Lifescience Market | 100 ul | EUR 438 |
 Trans110 Escherichia coli Strains |
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| S0046 | Lifescience Market | 100 ul | EUR 438 |
 MC1061 Escherichia coli Strains |
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| S0047 | Lifescience Market | 100 ul | EUR 438 |
 T1 Escherichia coli Strains |
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| S0048 | Lifescience Market | 100 ul | EUR 438 |
 EPI300 Escherichia coli Strains |
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| S0050 | Lifescience Market | 100 ul | EUR 438 |
 BW25113 Escherichia coli Strains |
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| S0051 | Lifescience Market | 100 ul | EUR 438 |
 BL21 Escherichia coli Strains |
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| S0053 | Lifescience Market | 100 ul | EUR 438 |
 EPI400 Escherichia coli Strains |
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| S0087 | Lifescience Market | 100 ul | EUR 438 |
 K802 Escherichia coli Strains |
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| S0089 | Lifescience Market | 100 ul | EUR 438 |
 JM108 Escherichia coli Strains |
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| S0105 | Lifescience Market | 100 ul | EUR 438 |
 HB2151 Escherichia coli Strains |
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| S0122 | Lifescience Market | 100 ul | EUR 438 |
) Recombinant Escherichia coli Deformylase Protein (aa 1-214) |
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| VAng-Lsx3100-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 2096.4 |
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Description: Escherichia coli Peptide deformylase, recombinant protein from E. coli. |
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Recombinant Escherichia coli Deformylase Protein (aa 1-214) |
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| VAng-Lsx3100-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 1436.4 |
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Description: Escherichia coli Peptide deformylase, recombinant protein from E. coli. |
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Recombinant Escherichia coli Deformylase Protein (aa 1-214) |
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| VAng-Lsx3100-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 986.4 |
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Description: Escherichia coli Peptide deformylase, recombinant protein from E. coli. |
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) Recombinant Escherichia coli trbH Protein (aa 1-239) |
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| VAng-Lsx3101-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Protein trbH, recombinant protein. |
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) Recombinant Escherichia coli trbF Protein (aa 1-126) |
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| VAng-Lsx3103-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Protein trbF, recombinant protein. |
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) Recombinant Escherichia coli srnB Protein (aa 1-68) |
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| VAng-Lsx3104-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Protein SrnB, recombinant protein. |
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) Recombinant Escherichia coli trbI Protein (aa 1-128) |
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| VAng-Lsx3105-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Protein trbI, recombinant protein. |
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) Recombinant Escherichia coli yjhE Protein (aa 1-82) |
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| VAng-Lsx3106-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Putative uncharacterized protein yjhE, recombinant protein. |
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) Recombinant Escherichia coli cvpA Protein (aa 1-162) |
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| VAng-Lsx3107-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Colicin V production protein, recombinant protein. |
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) Recombinant Escherichia coli trbA Protein (aa 1-115) |
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| VAng-Lsx3108-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Protein trbA, recombinant protein. |
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) Recombinant Escherichia coli rtcB Protein (aa 1-408) |
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| VAng-Lsx3109-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 860.4 |
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Description: Escherichia coli RNA-splicing ligase RtcB, recombinant protein. |
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Recombinant Escherichia coli rtcB Protein (aa 1-408) |
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| VAng-Lsx3109-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 594 |
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Description: Escherichia coli RNA-splicing ligase RtcB, recombinant protein. |
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Recombinant Escherichia coli rtcB Protein (aa 1-408) |
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| VAng-Lsx3109-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 424.8 |
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Description: Escherichia coli RNA-splicing ligase RtcB, recombinant protein. |
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) Recombinant Escherichia coli cea Protein (aa 1-522) |
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| VAng-Lsx3110-10g | Creative Biolabs | 10 µg | EUR 4665.6 |
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Description: Escherichia coli Colicin-E1, recombinant protein. |
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) Recombinant Escherichia coli ycfZ Protein (aa 1-262) |
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| VAng-Lsx3111-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Inner membrane protein ycfZ, recombinant protein. |
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) Recombinant Escherichia coli ytcA Protein (aa 27-91) |
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| VAng-Lsx3112-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Uncharacterized protein ytcA, recombinant protein. |
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) Recombinant Escherichia coli yfeZ Protein (aa 1-149) |
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| VAng-Lsx3113-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Inner membrane protein yfeZ, recombinant protein. |
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) Recombinant Escherichia coli yahG Protein (aa 1-472) |
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| VAng-Lsx3114-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Uncharacterized protein yahG, recombinant protein. |
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) Recombinant Escherichia coli ynbA Protein (aa 1-201) |
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| VAng-Lsx3115-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Inner membrane protein ynbA, recombinant protein. |
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) Recombinant Escherichia coli ygcG Protein (aa 17-290) |
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| VAng-Lsx3116-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli UPF0603 protein ygcG, recombinant protein. |
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) Recombinant Escherichia coli yjfZ Protein (aa 1-264) |
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| VAng-Lsx3117-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Uncharacterized protein yjfZ, recombinant protein. |
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) Recombinant Escherichia coli yjeO Protein (aa 1-104) |
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| VAng-Lsx3118-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Inner membrane protein yjeO, recombinant protein. |
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) Recombinant Escherichia coli yfdC Protein (aa 1-310) |
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| VAng-Lsx3119-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Inner membrane protein yfdC, recombinant protein. |
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) Recombinant Escherichia coli yedR Protein (aa 1-121) |
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| VAng-Lsx3120-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Inner membrane protein yedR, recombinant protein. |
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) Recombinant Escherichia coli yidI Protein (aa 1-149) |
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| VAng-Lsx3121-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Inner membrane protein yidI, recombinant protein. |
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) Recombinant Escherichia coli gltF Protein (aa 26-254) |
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| VAng-Lsx3122-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Protein gltF, recombinant protein. |
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) Recombinant Escherichia coli yjiK Protein (aa 1-286) |
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| VAng-Lsx3123-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Uncharacterized protein yjiK, recombinant protein. |
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) Recombinant Escherichia coli yafU Protein (aa 1-112) |
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| VAng-Lsx3124-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Putative inner membrane protein yafU, recombinant protein. |
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) Recombinant Escherichia coli ybhQ Protein (aa 1-136) |
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| VAng-Lsx3125-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Inner membrane protein ybhQ, recombinant protein. |
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) Recombinant Escherichia coli traQ Protein (aa 1-94) |
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| VAng-Lsx3126-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Protein traQ, recombinant protein. |
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) Recombinant Escherichia coli nrdD Protein (aa 1-712) |
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| VAng-Lsx3127-10g | Creative Biolabs | 10 µg | EUR 424.8 |
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Description: Escherichia coli Anaerobic ribonucleoside-triphosphate reductase, recombinant protein from E. coli. |
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) Recombinant Escherichia coli yhjK Protein (aa 1-662) |
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| VAng-Lsx3128-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Protein YhjK, recombinant protein. |
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) Recombinant Escherichia coli yigG Protein (aa 1-126) |
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| VAng-Lsx3129-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Inner membrane protein yigG, recombinant protein. |
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) Recombinant Escherichia coli yjdF Protein (aa 1-209) |
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| VAng-Lsx3130-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Inner membrane protein yjdF, recombinant protein. |
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) Recombinant Escherichia coli yedI Protein (aa 1-305) |
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| VAng-Lsx3131-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Inner membrane protein yedI, recombinant protein. |
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) Recombinant Escherichia coli ybdJ Protein (aa 1-82) |
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| VAng-Lsx3132-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Uncharacterized protein ybdJ, recombinant protein. |
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) Recombinant Escherichia coli yfgG Protein (aa 1-63) |
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| VAng-Lsx3133-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Uncharacterized protein yfgG, recombinant protein. |
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) Recombinant Escherichia coli ynjI Protein (aa 1-346) |
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| VAng-Lsx3134-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Inner membrane protein ynjI, recombinant protein. |
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) Recombinant Escherichia coli traD Protein (aa 1-717) |
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| VAng-Lsx3135-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Coupling protein TraD, recombinant protein. |
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) Recombinant Escherichia coli ybjE Protein (aa 1-299) |
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| VAng-Lsx3136-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Uncharacterized protein ybjE, recombinant protein. |
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) Recombinant Escherichia coli ydhU Protein (aa 1-261) |
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| VAng-Lsx3137-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Protein phsC homolog, recombinant protein. |
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) Recombinant Escherichia coli yhdV Protein (aa 1-73) |
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| VAng-Lsx3138-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Uncharacterized protein yhdV, recombinant protein. |
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) Recombinant Escherichia coli yegH Protein (aa 1-527) |
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| VAng-Lsx3139-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli UPF0053 protein yegH, recombinant protein. |
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) Recombinant Escherichia coli ygaM Protein (aa 1-113) |
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| VAng-Lsx3140-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Uncharacterized protein ygaM, recombinant protein. |
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) Recombinant Escherichia coli ybjM Protein (aa 1-125) |
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| VAng-Lsx3141-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Inner membrane protein ybjM, recombinant protein. |
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) Recombinant Escherichia coli elaB Protein (aa 1-101) |
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| VAng-Lsx3142-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Protein elaB, recombinant protein. |
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) Recombinant Escherichia coli aaeA Protein (aa 1-310) |
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| VAng-Lsx02138-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) p-hydroxybenzoic acid efflux pump subunit AaeA, recombinant protein. |
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) Recombinant Escherichia coli aaeX Protein (aa 1-67) |
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| VAng-Lsx02140-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli O1:K1 / APEC Protein AaeX, recombinant protein. |
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) Recombinant Escherichia coli acpP Protein (aa 1-78) |
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| VAng-Lsx02150-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3261.6 |
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Description: Escherichia coli (strain K12) Acyl carrier protein, recombinant protein. |
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Recombinant Escherichia coli acpP Protein (aa 1-78) |
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| VAng-Lsx02150-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2320.8 |
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Description: Escherichia coli (strain K12) Acyl carrier protein, recombinant protein. |
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Recombinant Escherichia coli acpP Protein (aa 1-78) |
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| VAng-Lsx02150-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1611.6 |
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Description: Escherichia coli (strain K12) Acyl carrier protein, recombinant protein. |
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) Recombinant Escherichia coli add Protein (aa 1-333) |
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| VAng-Lsx02151-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3952.8 |
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Description: Escherichia coli (strain K12) Adenosine deaminase, recombinant protein. |
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