Recombinant Proteinase K Solution
Recombinant proteinase K is a nonspecific serine protease that is useful for general protein digestion.
Proteinase K is a highly reactive serine protease that shows the ability to digest native proteins, thus inactivating enzymes such as DNase and RNase without resorting to a denaturation process. It is the most potent proteinase among all the proteinases characterized so far. It cleaves at the peptide bond adjacent to the carboxylic acid group of aliphatic, aromatic, or hydrophobic amino acids.
Recombinant proteinase K is used in the isolation or preparation of high molecular weight nucleic acids. It is highly pure and has a higher specific activity and is more stable at room temperature compared to native proteinase K.
Recombinant proteinase K is expressed from the yeast Pichia pastoris with a cloned gene encoding the endolytic protease of Engyodontium album (Tritirachium album).
Applications
• Recombinant proteinase K is extremely efficient on native proteins and therefore can be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines.
• It is used for the analysis of membrane structures by modifying proteins and glycoproteins on the cell surface.
• Particularly suitable for isolating nucleic acids for amplification reactions.
• Promotes cell lysis by activating a bacterial autolytic factor.
• Used to remove cell debris during colony lift preparation and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization.
Reconstitution Recommendations
For the preparation of the stock solution, we suggest reconstituting the lyophilized powder in a buffer (20 mM Tris, 1 mM CaCl2, 2% glycerol, pH 7.4).
Calcium is important for the thermostability of proteinase K and the pH value is critical for high solubility
The use of cryoprotectant glycerol to a final concentration of 50% in the stock solution is to prevent freeze / thaw damage. Proteinase K retained 99% of the activity after 12 freeze / thaw cycles in the stock solution. Avoid repeated freezing and thawing to avoid protein precipitation if glycerol is not added.
Proteinase K solution remains active in a wide pH range (4.0-12.5, often used in the 7.5-9.0 range) and in a wide temperature range of 25-75 ° C. The solution remains sterile and stable for at least 12 months at 4 ° C.
 DNA / RNA Damage Antibody |
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| abx443972-100ug | Abbexa | 100 ug | EUR 661.2 |
DNA / RNA Damage Antibody |
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| abx444253-100ug | Abbexa | 100 ug | EUR 661.2 |
DNA / RNA Damage Antibody |
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| abx444534-100ug | Abbexa | 100 ug | EUR 661.2 |
DNA / RNA Damage Antibody |
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| 20-abx444884 | Abbexa |
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DNA / RNA Damage Antibody |
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| abx440321-100ug | Abbexa | 100 ug | EUR 661.2 |
DNA / RNA Damage Antibody |
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| abx440602-100ug | Abbexa | 100 ug | EUR 661.2 |
DNA / RNA Damage Antibody |
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| abx440883-100ug | Abbexa | 100 ug | EUR 661.2 |
DNA / RNA Damage Antibody |
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| abx441164-100ug | Abbexa | 100 ug | EUR 661.2 |
DNA / RNA Damage Antibody |
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| abx441445-100ug | Abbexa | 100 ug | EUR 661.2 |
DNA / RNA Damage Antibody |
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| abx441726-100ug | Abbexa | 100 ug | EUR 661.2 |
DNA / RNA Damage Antibody |
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| abx442007-100ug | Abbexa | 100 ug | EUR 661.2 |
DNA / RNA Damage Antibody |
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| abx442288-100ug | Abbexa | 100 ug | EUR 661.2 |
 Monoclonal antibody for DNA/RNA Damage |
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| SMC-155C | Stressmarq | 0.025mg | EUR 228 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D | Stressmarq | 0.1mg | EUR 398.4 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-A390 | Stressmarq | 0.1mg | EUR 454.8 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 390. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-A488 | Stressmarq | 0.1mg | EUR 453.6 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 488. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-A565 | Stressmarq | 0.1mg | EUR 453.6 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 565. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-A594 | Stressmarq | 0.1mg | EUR 453.6 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 594. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-A633 | Stressmarq | 0.1mg | EUR 453.6 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 633. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-A655 | Stressmarq | 0.1mg | EUR 453.6 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 655. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-A680 | Stressmarq | 0.1mg | EUR 453.6 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 680. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-A700 | Stressmarq | 0.1mg | EUR 453.6 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 700. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-ALP | Stressmarq | 0.1mg | EUR 446.4 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Alkaline Phosphatase. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-APC | Stressmarq | 0.1mg | EUR 452.4 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with APC. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-APCCY7 | Stressmarq | 0.1mg | EUR 540 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with APC/Cy7. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-BI | Stressmarq | 0.1mg | EUR 448.8 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Biotin. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-DY350 | Stressmarq | 0.1mg | EUR 471.6 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 350. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-DY405 | Stressmarq | 0.1mg | EUR 457.2 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 405. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-DY488 | Stressmarq | 0.1mg | EUR 445.2 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 488. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-DY594 | Stressmarq | 0.1mg | EUR 447.6 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 594. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-DY633 | Stressmarq | 0.1mg | EUR 441.6 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 633. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-FITC | Stressmarq | 0.1mg | EUR 444 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with FITC. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-HRP | Stressmarq | 0.1mg | EUR 439.2 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with HRP. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-P594 | Stressmarq | 0.1mg | EUR 462 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with PE/ATTO 594. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-PCP | Stressmarq | 0.1mg | EUR 452.4 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with PerCP. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-RPE | Stressmarq | 0.1mg | EUR 450 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with RPE. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-STR | Stressmarq | 0.1mg | EUR 451.2 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Streptavidin. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155S | Stressmarq | 0.012mg | EUR 78 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated. |
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 DNA Damage Quantification Kit |
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| 55R-1345 | Fitzgerald | 25 assays | EUR 1012.8 |
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Description: Assay Kit for Quantification of DNA Damage in the research laboratory |
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 DiscoveryProbe? DNA Damage/DNA Repair Library |
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| L1033-.1 | ApexBio | 100 uL/well(10 mM solution) | EUR 4666.8 |
DiscoveryProbe? DNA Damage/DNA Repair Library |
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| L1033-.25 | ApexBio | 250 uL/well(10 mM solution) | EUR 8286 |
DiscoveryProbe? DNA Damage/DNA Repair Library |
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| L1033-5 | ApexBio | 5 mg/well | EUR 10806 |
) OxiSelect Nitrosative DNA/RNA Damage ELISA Kit (8-Nitroguanine Quantitation) |
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| STA-825 | Cell Biolabs | 96 assays | EUR 957.6 |
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Description: The OxiSelect Nitrosative DNA/RNA Damage ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 8-nitroguanine in urine, serum, or plasma samples. |
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OxiSelect Nitrosative DNA/RNA Damage ELISA Kit (8-Nitroguanine Quantitation) |
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| STA-825-5 | Cell Biolabs | 5 x 96 assays | EUR 3888 |
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Description: The OxiSelect Nitrosative DNA/RNA Damage ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 8-nitroguanine in urine, serum, or plasma samples. |
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 DNA/RNA Damage Quantification Kit (Colorimetric)) EpiQuik Nitrosative (8-Nitroguanine) DNA/RNA Damage Quantification Kit (Colorimetric) |
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| P-6009-48 | EpiGentek | 48 Assays | EUR 486.2 |