SPHEROTM Ultra Rainbow Fluorescent Particles - IMMPACT:Initiative for Cell Mortality Programme Assessment.

Consists of a single peak for optical alignment of any
fl ow cytometer in all channels from UV to Far Red
• Determine if the fl ow cell is clean and without fl uidic blockage
• Measures the coeffi cients of variation (CVs), peak channels, and histogram distribution to determine the functionality of fl ow cytometers.

New fl ow cytometers, with fl uorescent channels from the UV to Far Red, and corresponding fl uorescent conjugates are now available. As a result, we have developed the Ultra Rainbow Fluorescent Particles with enhanced UV and Far Red fl uorescence intensity. The Ultra Rainbow Fluorescent Particles contain a single peak and are designed for checking the optical alignment of any fl ow
cytometer in all channels.

SPHEROTM Ultra Rainbow Fluorescent Particles for Beckman Coulter Cytometers

Consists of a single peak for performance verification of Beckman Coulter flow cytometers
• Determine cleanliness of the flow cell and fluidics

These Rainbow Calibration Particles are a mixture of 3.0 – 3.4 μm particles, dyed to six different fluorescent intensities. As their excitation range is 365 – 650 nm, they can be used with most lasers. However, they are not for use with the UV laser. The emission spectra of these rainbow particles is compatible with many common fluorophores used in immunofluorescent staining with flow cytometric analysis.

SPHERO Ultra Rainbow Fluorescent Particles

A single population of Rainbow Fluorescent Particles with enhanced UV and Far Red fluorescence intensity
Spherotech offers a wide variety of particles for determining if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

The vial contains a mixture of 3.0 – 3.4 µm Rainbow Particles that are dyed to eight different fluorescent intensities. Every Rainbow Particle contains a mixture of fluorophores that are excited at any wavelength from 365 – 650 nm. The Rainbow Particles have emission spectra compatible with many common fluorophores used for immunofluorescent staining with flow cytometric analysis.

Recommended Assay Procedures

This particle mixture (~10×10^6 particles/ml) is useful for routine calibration of flow cytometers. Before use, resuspend the particles by vortexing. Dilution of 3 – 5 drops of particles to 1 ml of sheath fluid will provide an adequate number of particles for flow cytometric analysis.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding them to avoid the accumulation of potentially explosive deposits in plumbing.
Cy is a trademark of GE Healthcare.
Please refer to resources for technical protocols.

Rainbow Fluorescent Particles
RFP-35-5	Spherotech	5 mL	EUR 364.8
Description: Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Rainbow Fluorescent Particles
RFP-38-5	Spherotech	5 mL	EUR 188.4
Description: Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Rainbow Fluorescent Particles
RFP-50-5	Spherotech	5 mL	EUR 376.8
Description: Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Rainbow Fluorescent Particles
RFP-60-5	Spherotech	5 mL	EUR 376.8
Description: Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Rainbow Fluorescent Particles
RFP-70-5	Spherotech	5 mL	EUR 376.8
Description: Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Supra Rainbow Fluorescent Particles
SRCP-35-2A	Spherotech	2X1 mL	EUR 346.8
Description: Supra Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Supra Rainbow Fluorescent Particles
SRCP-35-5A	Spherotech	5X1 mL	EUR 700.8
Description: Supra Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Supra Rainbow Fluorescent Particles
SRCP01-35--10A	Spherotech	10X1 mL	EUR 194.4
Description: Supra Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Supra Rainbow Fluorescent Particles
SRCP01-35--2AK	Spherotech	2X1 mL	EUR 486
Description: Supra Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Supra Rainbow Fluorescent Particles
SRCP10-35--2A	Spherotech	2X1 mL	EUR 1497.6
Description: Supra Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.