Tag: western blot method
A linear epitope coupled to DsRed provides an affinity ligand for the capture of monoclonal antibodies
Monoclonal antibodies (mAbs) dominate the market for biopharmaceutical proteins because they provide active and passive immunotherapies for many different diseases. However, for most mAbs, two expensive manufacturing platforms are required. These are mammalian cell cultures for upstream production and Protein A chromatography for product capture during downstream processing. Here we describe a novel affinity ligand…
Read MoreUtilization of a direct sandwich ELISA kit for rapid diagnosis of adenovirus hemorrhagic cystitis in a patient with adult T-cell leukemia
We report on a case of adenovirus hemorrhagi cystitis that developed in a 49-year-old woman during intensive chemotherapy for adult T-cell leukemia. Although rapid etiologic diagnosis is essential for the effective management of viral hemorrhagic cystitis, the isolation of adenovirus from urine is often too time-cousuming. We detected the adenovirus antigen in the patient’s urine…
Read MoreHigh-Throughput Screening of a Promoter Library Reveals New Persister Mechanisms in Escherichia Coli
Persister cells are a small subpopulation of phenotypic variants that survive excessive concentrations of bactericidal antibiotics. Their survival mechanisms will not be heritable and will be fashioned stochastically or triggered by environmental stresses equivalent to antibiotic therapy. On this examine, high-throughput screening of an Escherichia coli promoter library and subsequent validation experiments recognized a number…
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Combined p53 and Bcl2 Immunophenotypes in Prognosis of Vietnamese Invasive Breast Carcinoma: A Single Institutional Retrospective Analysis
Background: Aberrant of p53 and Bcl2 genes trigger adjustments within the amount and high quality of their proteins and contribute to the pathogenesis of some most cancers varieties together with breast most cancers. Expression of p53 and Bcl2 had been related to antagonistic medical outcomes in breast most cancers. Function: To foretell the survival outcomes of invasive…
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TOM20-mediated transfer of Bcl2 from ER to MAM and mitochondria upon induction of apoptosis
On this work, we have now explored the subcellular localization of Bcl2, a significant antiapoptotic protein. In U251 glioma cells, we discovered that Bcl2 is localized primarily within the ER and is translocated to MAM and mitochondria upon induction of apoptosis; this mitochondrial switch was not restricted to the demonstrator cell line, even when cell-specific…
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